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DNeasy® 96 Plant Protocol for Isolation of DNA from Fresh Plant Leaves Using the Mixer Mill MM 300 一、Important notes before starting （使用前的重

1.没有回收到 DNA 片段 如在洗脱后,发现洗脱液中没有 DNA 片段,请检查是否按瓶身标签,在洗涤液中加入了无水乙醇. 2.提取率低 (1)融胶液为酸性缓冲液,如融胶后,其pH 值升高将影响提取得率.请加入0.1 倍体积的3M乙酸钾(pH5.0). (2)长时间使用后

Silica Suspension: add 2 g of silica to 15 ml of H2O wash 3x by centifugation at 2000 x g for 2 min estimate vol of silica and resuspend

PROTOCOL TO EXTRACT DNA FROM PARAFFIN BLOCKS 1.Cut 10-20X10μm sections of formalin fixed paraffin samples into eppendorf tubes. 2.Add 1 ml

Materials: TE buffer 10% (w/v) sodium dodecyl sulfate (SDS) 20 mg/ml proteinase K phenol\chloroform (50:50) isopropanol 70% ethanol 3

1. Grow 10-ml yeast cultures overnight to saturation in appropriate media at 30℃. 2. Spin cells down for 5min at 1,500rpm (Beckmann), and r

Purpose: Purification of oligonucleotides (which have already been purified by reverse phase) can increase the efficiency of site directed m

Add NH4Ac (10 M stock or solid) to the sample for a final concentration of 2.5 M, mix (spin at 4℃, transfer the supernatant to a new tube; o

This is a scaled up version of the Baron protocol which has been modified to achieve purity comparable to CsCl preps. I have not sorted out

Phenol (removes protein) 1.add equal volume of Phenol (= tris-saturated Phenol-Chloroform-Isoamyethanol) 2.vortex 3.spin 2 minutes at 120