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1.Separate DNA fragments in an agarose gel cast with 0.5 mg/ml Ethidium bromide.Locate bands with a hand-held long-wave UV lamp. 2.Slice th

1.Add an equal volume (equal to sample volume)of P/C to sample. 2.Mix (shake,don't vortex). 3.Take aqueous (upper)layer.(If dirty sample,r

Isolation of Mouse genomic DNA 1.Kill mouse by cervical luxation and dissect liver.Add to a 50ml tube and place on liquid nitrogen.Grind to

1.Prepare or obtain Buffered phenol,pH 8.Add 2 crystals of 8-Hydroxyquinoline to prevent oxidation.This also identifies the organic phase as

This is a modification of the procedure in Short Protocols (1-41,1-45) 1.Prepare a 50 ml liquid lysate: A.mix 2xlO8 E.coli cells with 100μ

Pick colonies into 0.5ml of SD-Leu (or other appropriate SD medium) Vortex for 1min Leave to grow O/N for 18-24h at 30℃, 230-250rpm (best

DNA Fragment Purification from Agarose or Acrylamide For fragments from 200 bp to 10 kb the agarose purification is ideal.For smaller fragm

1.grow plants in trays of 96 and leave two spots open (for the PCR controls) 2.harvest 1 to 2 young and green leaves (1cm2 /plant, at roset

1.加入solution.III，经10分钟离心后细菌沉淀怎么不结实，有的漂浮在液面，有的贴在离心管壁上，一摇晃即破碎脱落下来？ 细菌的用量太少，导致产生的沉淀主要是盐分的沉淀，因为缺少变性的细菌蛋白和细菌基因组DNA 的缠绕，沉淀就显得不结实。 解决方法：将细菌的用量增加。

Jacks Lab,Center for cancer research,MIT 1.Each tail should be in a clean eppendorf tube. 2.Add 500µl of tail lysis buffer containing Prot