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Isolation of Mouse genomic DNA Kill mouse by cervical luxation and dissect liver. Add to a 50ml tube and place on liquid nitrogen. Grind to

Important Notes Before Starting • New users are strongly recommended to read the QIAGEN handbook before starting the procedure. •Before us

1. Grind 20-50 frozen flies with a mortar and pestle in N2(l) and suspend in 2 ml Lysis Buffer. 2.Add Proteinase K to 100 mg/ml; incubate 1

Steve Hahn Last modified 10/16/98 Purpose: Purification of oligonucleotides (which have already been purified by reverse phase) can increa

Abstract: The “Freeze squeeze” method is a cost effective and technically simple procedure to isolate and prepare PCR products for DNA seque

Submission Details: *Dr. Simon Dawson*Department of Biochemistry*University of Nottingham*U.K.*17:03*22/1/96. Database: Method/Protocol-->Sp

Abstract: It is very difficult to isolate Rhizobium DNA due to the gum production by the organism. Hence we have designed a protocol for eff

一、实验目的 学习和掌握DNA的微量提取法。 二、实验原理 小麦黄化苗经液氮研磨，可使细胞壁破裂，加入去污剂（如SDS），可使核蛋白体解析，然后使蛋白和多糖杂质沉淀，DNA进入水相，再用酚、氯仿抽提纯化。本实验采用SDS法，其主要作用是破膜。SDS属阴离子去污剂，要溶解膜蛋

This procedure allows the concentration of DNA samples from dilute solution and the removal of unwanted salts from DNA samples. Materials

In the hood: 96 well dish with bacteria titertech microtubes glass pipette Remove colonies from each well using the titertech and place