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Purpose DNA extraction without phenol extraction and centrifugation. Procedure 1. Lysis: the lysis buffer (usually 0.5ml) is added to the

David Harry Institute of Forest Genetics USDA Forest Service Pacific Southwest Research Station August 26, 1993 Background : There are

Preparation of Sonicated Salmon Sperm DNA Procedure: 1) Use Pharmacia #27-4564-01. With clean flamed scissors and forceps, weigh 0.25 g/50

1. Transfer plugs of mycelial margin to 0.1 P plates. Allow to grow to fill plate (about one week at 21°). 2. Macerate two plates of myceli

A practical method for the preparation of total DNA from filamentous fungi M.I. Borges, M.O. Azevedo, R. Bonatelli, Jr., M.S.S. Felipe and

内 容 提 要 以枯草芽孢杆菌为材料，采用CTAB法（十六烷基三甲基溴化铵）提取枯草芽孢杆菌（Bacillus subtilis）的基因组DNA作为基因扩增的模板，并测定其含量和纯度。 本实验要求：提取高质量的基因组DNA为下一步的基因操作准备。 原 理 高纯度、高分子量

实验目的 将复杂的细胞分子混合物加入有机溶媒萃取以除去蛋白质及其它成分，就可以纯化DNA。一般常用酚及氯仿 (phenol/chloroform)可使蛋白质变性(denaturaion)的特性来进行萃取的步骤，DNA和RNA不溶于有机溶媒中，而溶于水层。另外，分子选殖(mole

1.Add 750μl Reagent B (50mM Tris [pH8],10mM EDTA,100mM NaCl,1% [w/v] SDS)and 50μl 100mg/ml proteinase K to each spleen. 2.Incubate overnigh

Protocol for extracting DNA from ES Cells,starting from the 96-well plate but processing in an eppendorf tube to recover more of the DNA.NOT

Abstract: Single strand conformational polymorphism (SSCP)is the most widely used PCR-based methods for point mutation detection. The abnorm