• DNA末端修饰原理

    Chapter 4 replication DNA replication DNA damage and repair Reverse transcription RNA replication DNA复制的基本特点 半保留复制(self-conservative r
    2010-03-11
  • An inexpensive alternative to glassmilk for DNA purification

    Preparation of Silica 1. Suspend 5 g of silica (Sigma, S-5631) in 50 ml of PBS. 2. Allow the silica to settle for 2h. 3. Discard the supe
    2010-03-11
  • 细菌总DNA的提取和鉴定

    【目的和要求】 1.学会CTAB法提取细菌总DNA。 2.掌握DNA的琼脂糖凝胶电泳法。 【实验原理】 DNA在生物体内是与蛋白质形成复合物的形式存在的,因此提取出脱氧核糖核蛋白复合物后,必须将其中蛋白质去除。CTAB(溴代十六烷基三甲胺)是一种去污剂,它能与核酸形成复合
    2010-03-11
  • Methods for DNA isolation(DNA分离方法)

    (adapted from Bruce A.Roe,Department of Chemistry and Biochemistry,The University of Oklahoma,Norman,Oklahoma 73019 broe@ou.edu) A.Large sc
    2010-03-11
  • 质粒提取(plasmid prep)简介及问题分析

    一、导论 质粒提取的原理:转自复旦大学一位老师的帖子,后面是方法介绍 碱裂解法从大肠杆菌制备质粒,是从事分子生物学研究的实验室每天都要用的常规技术。可是我收研究生十几年了,几乎毫无例外的是我那些给人感觉什么都知道的优秀学生却对碱法质粒抽提的原理知之甚少。追其原因,我想大概是因为
    2010-03-11
  • 脉胞菌DNA的快速提取

    An Ultra-fast method of DNA extraction from Neurospora Edward B. Cambareri and John A. Kinsey - Department of Microbiology, Molecular Genet
    2010-03-11
  • Triton-Prep Method for bacterial DNA Purification

    1.Grow 5 ( large scale-15ml culture). Harvest in single eppendorf tube (or 15 ml disposable tube). 2.Resuspend pellet with 300μl STET buffe
    2010-03-11
  • 细菌基因组DNA的微量提取法

    本方法通过SDS裂解细胞,蛋白酶降解蛋白,CTAB去除多糖成分 一:仪器:同方法一 二:试剂: TE、TAE缓冲液;10%SDS;5MNaCL; 20mg/ml蛋白酶K;CTAB/NaCL溶液(10% CTAB,0.7M NaCL 4.1gNaCL 溶于80ml水中,缓
    2010-03-11
  • Extraction of genomic DNA from whole blood

    Abstract: The protocol is simple and fairly rapid.It does not require the use of organic solvents but rather utilizes salt extraction to pre
    2010-03-11
  • DNA Preparation from Adherent Cells

    Section of Cancer Genomics,Genetics Branch,NCI National Institutes of Health Reagents Chloroform Mallinckrodt,cat.4440 EDTA,0.5 M Etha
    2010-03-11