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(adapted from Bruce A.Roe,Department of Chemistry and Biochemistry,The University of Oklahoma,Norman,Oklahoma 73019 broe@ou.edu) Phenol ext

Isolation of DNA from Agarose Gels (Paper Slurry Method) This procedure isolates DNA from agarose gels by filtration through a filter-paper

A protocol / method / schedule /procedure for extraction / isolation of both DNA and RNA from the same material typically plant leaf / leave

1.Pour a vertical acrylamide gel using TEA buffer.A 4 % non denaturing gel is correct for most applications. 2.Run out DNA fragments.For fr

This procedure was originally developed for Listeria monocytogenes but has worked well with other Gram+ bacteria we've tried. 1.Pellet cell

1.Crash Individual sand flies in 0.5 ml lysis buffer (50 mM NaCl ,10 mM EDTA,50 mM Tris-HCl pH 8).2.Incubate The sand fly homogenates with 1

Important : Extract the DNA within one week of receiving samples.Samples that have been processed should be frozen to prevent degradation.Fr

[原理] 本法用含EDTA的溶液洗涤细胞，SDS（十二烷基硫酸钠）破碎、裂解细胞，消化蛋白质，使核蛋白解聚并使细胞内的DNA酶失活，用酚、氯仿变性蛋白质并去除杂质，最后用乙醇沉淀得到纯化的DNA。 [试剂] 1.裂解缓冲液 2.苯酚-氯仿溶液 3.氯仿-异戊醇溶液 4

实验步骤： 1、待回收的DNA段经电泳分离后，从琼脂糖凝胶上切下所需DNA段，放在1.5ml EP管中； 2、加入3 倍（请准确估量凝胶的体积）体积的溶胶液（以100μl 体积胶加入300μl 溶胶液为一次标准反应），室温下放置5分钟或50℃ 保温3分钟，其间轻摇EP管几次使

1.Excise band of interest from a TAE gel; estimate volume by weighing the gel slice. 2.Dissolve the gel slice in 3 volumes of NaI (from Gen