• PAGE(聚丙烯酰胺凝胶电泳)实验

    【实验原理】 聚丙烯酰胺凝胶电泳(polyacryamide gel electropHoresis, PAGE)是由丙烯酰胺单体和交联剂甲叉双丙烯酰胺在催化作用下形成的三维网状结构物质。在不连续聚丙烯酰胺凝胶电泳中,凝胶的制作是分层进行的,因此凝胶不仅有分子筛效应,还具有浓缩
    2010-04-09
  • r-hGH中间体SDS-PAGE电泳标准操作规程(SOP)

    目的:规范生长激素中间体的电泳检测 一.仪器装置 1.电泳仪 2.夹心式垂直电泳槽 3.玻璃板:长14.5×11.5×2mm、短14.0×11.5×2mm 4.可调微量移液器 5.微量进样器 6.转移脱色摇床 二.试剂配制 1.丙烯酰胺液(30%T/2.7%C)
    2010-04-09
  • SDS-PAGE(SDS-聚丙烯酰氨凝胶电泳)

    This is a lab manual for discontinuous (Laemmli) denaturing SDS-PAGE and Coomassie staining. It is based on the Mini-PROTEIN 3 Cell Instruct
    2010-04-09
  • SDS-PAGE Gel Electrophoresis

    The goal of this lab is to separate your isolated proteins using sodium dodecylsulfate (SDS) polyacrylamide gel electrophoresis (PAGE) and s
    2010-04-09
  • SDS-PAGE and Molecular Systematics

    Reconstructing Fish Phylogeny What is SDS-PAGE? SDS (Sodium dodecyl sulphate) is a detergent used to denature proteins PAGE (Polyacrylami
    2010-04-09
  • 蛋白电泳NuPAGE Gel Electrophoresis

    NuPAGE Gels A gel electrophoresis system used for SDS-PAGE protein analysis.The gels are made up of Bis-Tris-HCl (pH 6.4)polyacrylamide and
    2010-04-09
  • Silver Staining of SDS-PAGE Gels(Ⅰ)

    1.Rinse gel on glass plate briefly with distilled water. 2.Wash gel in fresh 50% ETOH-12% HAc,with shaking,3 times,1 hour each.(Fix IEF gel
    2010-04-09
  • SDS-PAGE的影响因素

    1. 带电颗粒的性质 净电荷多少、颗粒大小及形状。一般净电荷多,直径小而且近于球状,则泳动速度快,反之则慢。 2. 电场强度(电位梯度) 指单位长度(cm)支持物体上的电位降,它对泳动度起着十分重要的作用。一般电场强度越高,带电颗粒移动速度越快。根据电场强度可将电泳分为低压
    2010-04-09
  • SDS-PAGE实验方法

    试剂: 1. 5x样品缓冲液(10ml):0.6ml 1mol/l的Tris-HCl(pH6.8),5ml 50%甘油,2ml 10%的SDS,0.5ml巯基乙醇,1ml 1%溴酚蓝,0.9ml蒸馏水。可在4℃保存数周,或在-20℃保存数月。 2. 凝胶贮液:在通风橱中,称取
    2010-04-09
  • SILVER STAIN OF PROTEIN GELS(Ⅱ)

    1.Fix gel in 50% methanol for greater than 1 hour rinse gel in dH2O 3 times 5 minutes each place back in 50% methanol for 1 hour. 2.Stain g
    2010-04-09