• SOUTHERN BLOT protocol

    1. Run the gel as normal. Often for genomic southerns it is desirable to run long gels (18cm) over 4-6hrs. 2. Photograph the gel with a rul
    2010-03-07
  • Southern杂交分析原理和操作

    【原理】 Southern杂交是分子生物学的经典实验方法。其基本原理是将待检测的DNA样品固定在固相载体上,与标记的核酸探针进行杂交,在与探针有同源序列的固相DNA的位置上显示出杂交信号。通过Southern杂交可以判断被检测的DNA样品中是否有与探针同源的片段以及该片段的长度
    2010-03-07
  • Southern印迹技术原理和操作步骤

    实验原理: Southern印迹是将DNA片断从电泳凝胶上直接转移至膜支持物(如硝酸纤维素膜、尼龙膜)上,使DNA片断固定的技术。先将DNA经限制性内切酶消化成一系列片段,进行琼脂糖凝胶电泳,各片段因分子量不同而彼此分开,然后经碱处理凝胶,使DNA的片段被变性、中和并通过毛细作
    2010-03-07
  • SOUTHERN BLOT的操作步骤

    1. Run the gel as normal. Often for genomic southerns it is desirable to run long gels (18cm) over 4-6hrs. 2. Photograph the gel with a rul
    2010-03-07
  • Southern blot实验方法与步骤

    用于检测重组DNA,也可分析DNA样品中是否有与探针序列同源的DNA片段。用于基因诊断,也可验证检测片段的分子量大小。将基因组DNA经限制性内切酶酶切,进行琼脂糖电泳,把分离后定位在凝胶上的不同分子量的DNA经碱变性处理,将凝胶中变性的DNA转移至一固相支持滤膜。利用标记的某一D
    2010-03-29
  • Screen ES cells by Southern Blot

    Digest DNA in 96-well plate To each well add: 4ul 10Xbuffer 4ul Enzyme 0.4ul Spermidine(0.4M) 31.6ul H2O 37‡C 19h, then add 4ul loadin
    2010-03-08
  • Southern Blot Hybridization

    blotting 1. Run gel to desired distance. 2. Visualize gel on UV box. Note the marker bands by: a) using a fluorsecent ruler and photograp
    2010-03-08
  • 基因组DNA Southern杂交操作步骤

    1)基因组DNA Southern印迹的制备 预备 1.用适当的限制性内切酶消化基因组DNA样品(10μg)。 2.进行琼脂糖凝胶电泳。一般用0.7~1.0%的琼脂糖凝胶分离基因组DNA,它在1~15kb的范围内有较好的分辨率,可选用TBE或TAE缓冲液。琼脂糖凝胶电泳需在
    2010-03-08
  • Southern Hybridization Protocols

    Two protocols are given here. The first one is used for BAC library screening on filters , although is also suitable for Transfer hybridiza
    2010-03-08
  • Alkaline Southern Blotting Procedure

    Author: Suzanne Gerttula Date: March 14,1994 Digest between 1 and 10 ug Genomic DNA. I digest at 37 C for about six hours over the course
    2010-03-08