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1.Safety precautions For general safety in laboratory work, consider the general document RWS 004 on environmental safety and work security

Description: The RT-PCR Primer and Control Set is provided for use as a positive control in RT-PCR applications. It contains human β-actin

Overview The use of reverse transcriptases to perform first-strand cDNA synthesis has had a huge impact on the study of biological processe

We will provide both one-step and two-step protocols for RT-PCR. We recommend the two-step protocol for this class. In the one-step protoco

本文讨论的范围包括RNA酶保护分析，northernblot，原位杂交，半定量RT-PCR和定量RT-PCR。本文不谈具体protocol，是因为各种书籍和kit说明书上都有，主要说一些原则，而且大部分是失败和成功的经验，还有小组讨论结果以及各种书里七零八落看的内容，希望对大家有

RT-PCR Analysis Solutions 10X RT Buffer 10X PCR Buffer 100 mM Tris pH 9.0 500 mM KCl 1% Triton X-100 25 mM MgCl2 use at a concentrat

Application: Quantitative RT-PCR is used to quantify mRNA in both relative and absolute terms. It can be applied for the quantification of m

RT-PCR can be performed as either a two-step or an one-step procedure. Each has certain advantages: 1. Two-step procedures A. Two tube, tw

RT-PCR lab You have a cell…is a certain gene on (by “on,” we mean active and producing mRNA?)? If a certain gene is on when the cell divid

A frequent cause of concern among investigators performing quantitative RT-PCR is inaccurate data due to DNA contamination in RNA preparatio