Description:
The RT-PCR Primer and Control Set is provided for use as a positive control in RT-PCR applications. It contains human β-actin sense and anti-sense primers (control primers) and total HeLa RNA sufficient for 20 amplification
reactions of 50 μl each. The control primers are designed from the human β-actin mRNA gene and produce a 353-bp RT-PCR product (1).
Quality Control:
The product is tested functionally using 10 pg of total HeLa RNA as the template for amplification of a 353-bp segment of β-actin mRNA (40 cycles).A minimum of 25 ng of the RT-PCR product was obtained under these
conditions.
Limited Label License No. 4:
This product is optimized for use in the Polymerase Chain Reaction (PCR) covered by patents owned by Roche Molecular Systems, Inc. and F. Hoffmann-La Roche, Ltd. (“Roche”). No license under these patents to use the PCR process is conveyed expressly or by implication to the purchaser by the purchase of this product. A license to use the PCR process for certain research and development activities accompanies the purchase of certain reagents from licensed
suppliers such as Invitrogen, when used in conjunction with an Authorized Thermal Cycler, or is available from Applied Biosystems. Further information on purchasing licenses to practice the PCR process may be obtained by contacting the Director of Licensing at Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404 or at Roche Molecular Systems, Inc.,1145 Atlantic Avenue, Alameda, California 94501.
Procedure:
This protocol uses components from the SuperScript
