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S1 Nuclease Protection Assay
S1 Nuclease Protection Assay End-label oligo (20-25 mer) 4 µl 5X T4 Kinase Buffer 5 µl [gamma-32P] ATP (7000 Ci/mmol) 10 µl water + olig
2010-05-20
In situ immunodetection of 5-methylc
In situ immunodetection of 5-methylcytosine (5-mC) on squashed cells using anti-methylcytosine Cross-references are to Schwarzacher & Heslo
2010-05-20
Bisulfite Modification of DNA
Source: Protocol Online Abstract: Modifying DNA using sodium bisulfite to convert unmethylated cytosines to uracils and subsequently detect
2010-05-20
Methylation analyis using restriction&nbs
Methylation analyis using restriction enzyme digestion This method has some weak points.First is that not all CG are located within CCGG se
2010-05-20
Bisulfite Treatment of DNA
Adapted from Frommer et.al.* 1.Dilute DNA (up to 2 mg) into 50 ml with distilled H2O. 2.Add 5.5 ml of 2M NaOH. 3.Incubate at 37℃ for 10 m
2010-05-20
Methylation-Specific PCR
Protocol written by James Herman* Methylation-specific PCR (MSP) is a simple rapid and inexpensive method to determine the methylation stat
2010-05-20
DNA-Methyltransferase Assay
This protocol was written by Jean-Pierre Issa,based on Adams et al.* Kam-Wing Jair has made some useful shortcuts that work well if you are
2010-05-20
表观遗传学关于DNA甲基化
表观遗传学是研究表观遗传变异的遗传学分支学科从目前的研究来看,X 染色体剂量补偿、DNA 甲基化、组蛋白密码、基因组印记、表观基因组学和人类表观基因组计划等问题都是表观遗传学研究的内容。其中甲基化是基因组DNA 的一种主要表观遗传修饰形式,是调节基因组功能的重要手段。在脊椎动物中
2010-07-22
Comparative Genomic Hybridization (CGH)
Protocol For the original protocol describing CGH see the article by Kallioniemi et al . For an example of how this technique has been util
2010-03-07
FINGERPRINTING PROTOCOL (AGAROSE GEL)
FINGERPRINTING PROTOCOL (AGAROSE GEL) * Restriction digests consist of: 15.75 ml ddH2 O 1 µl 10 X buffer B (Boehringer Mannheim) 0.25 µl
2010-03-07
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