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S1 Nuclease Protection Assay End-label oligo (20-25 mer) 4 µl 5X T4 Kinase Buffer 5 µl [gamma-32P] ATP (7000 Ci/mmol) 10 µl water + olig

In situ immunodetection of 5-methylcytosine (5-mC) on squashed cells using anti-methylcytosine Cross-references are to Schwarzacher & Heslo

Source: Protocol Online Abstract: Modifying DNA using sodium bisulfite to convert unmethylated cytosines to uracils and subsequently detect

Methylation analyis using restriction enzyme digestion This method has some weak points.First is that not all CG are located within CCGG se

Adapted from Frommer et.al.* 1.Dilute DNA (up to 2 mg) into 50 ml with distilled H2O. 2.Add 5.5 ml of 2M NaOH. 3.Incubate at 37℃ for 10 m

Protocol written by James Herman* Methylation-specific PCR (MSP) is a simple rapid and inexpensive method to determine the methylation stat

This protocol was written by Jean-Pierre Issa,based on Adams et al.* Kam-Wing Jair has made some useful shortcuts that work well if you are

表观遗传学是研究表观遗传变异的遗传学分支学科从目前的研究来看,X 染色体剂量补偿、DNA 甲基化、组蛋白密码、基因组印记、表观基因组学和人类表观基因组计划等问题都是表观遗传学研究的内容。其中甲基化是基因组DNA 的一种主要表观遗传修饰形式,是调节基因组功能的重要手段。在脊椎动物中

Protocol For the original protocol describing CGH see the article by Kallioniemi et al . For an example of how this technique has been util

FINGERPRINTING PROTOCOL (AGAROSE GEL) * Restriction digests consist of: 15.75 ml ddH2 O 1 µl 10 X buffer B (Boehringer Mannheim) 0.25 µl