首页/产业动态/

This procedure is used to analyze inserts in pUC-derived plasimids. 1. Suspend E. coli colonies harbouring plasmids in 50 microliters of TE

Richard Powell Department of Microbiology, National University of Ireland, Galway, Ireland Frank Gannon European Molecular Biology Laborat

Prepare a ligation mix: Ligation Mix (2x) 10x ligase buffer 1.0 ml digested vector (0.1 mg/ml) 1.0 ml H2 O 6.0 ml total 8.0 ml di

1、分别设立两个反应，用适当的限制性内切核酸酶消化1－10μg质粒DNA和外源DNA片段，使它们能产生平末端。 2、苯酚：氯仿抽提与乙醇沉淀法纯化出已被消化的载体和外源DNA片段。 3、分别用TE（pH 8.0）重新溶解纯化出的两种DNA沉淀，使终浓度为100 ng/ml。假

1、Oligo design （1）The 5' end (the homology arm) - choose 42 or more (we usually choose about 50) nts for the homology arms from the target

Serial Analysis of Gene Expression (SAGE) SAGE is a powerful tool that allows the analysis of overall gene expression patterns with digital

一、DNA 的酶切与连接 （1）酶切反应：同质粒DNA 的鉴定，只不过是质粒DNA 换为载体DNA 。若大量酶切，则成比例增加。 （2）加2倍体积的预冷无水乙醇和1/10体积的3mol/l NaAc混匀，-20℃2h以上。 （3）15000rpm离心15min，弃上清。

单拷贝克隆产量低，高拷贝克隆稳定性差，一直让研究者在克隆 表达时难以选择。蛋白的表达就有诱导表达系统，可以实现人为地控制蛋白的表达时间和表达量——现在连质粒的拷贝数可以借助诱导的方法来人为控制了！Epicentre的专利技术——CopyControl克隆 系统能够让您共享单拷贝和

Introduction This manual is a compilation of many of the everyday methods used in the average molecular biology laboratory, with emphasis o

DNA WALKING REFERRENCEKi-Bum Park and Suk-Heung Oh(2007)Cloning, sequencing and expression of a novel glutamate decarboxylase gene from a ne