Blotting with BSA
1. Blot in 5% mile/ 1X PBST (or 1X TBST) --60'/Rt
2. Wash 2X for 5' in 1XPBST
3. 10Ab in 1% BSA/1X PBST --60'/Rt
4. Wash PBST 15', then 3x for 5' each in 1X PBST
5. 20Ab in 1% BSA/1X PBST --60'/Rt (aHRP)--
6. Wash 15' with 1X PBST then 3x with PBST for 5' @
7. Chemiluminescence: Mix 1:1 NEN reagents. Add to Mb --1'/shaking. Blot excess
8. Put between sheets (page protector) and expose film
