Running Protein Gels
Solutions
10X Running Buffer (0.25 M Tris, 1.92 M glycine, 1% SDS)
121 g Tris
577 g glycine
40 g SDS
ddh20 to 4 L (check pH at 1:10 dilution (pH=~8.8)).
5X Sample Buffer (0.3125 M Tris pH 6.8, 10% SDS, 50% glycerol, 0.005% bromophenol blue, 25% 2-mercaptoethanol)
1.56 ml 2M Tris pH 6.8
1 g SDS
5 ml glycerol
0.2 ml 0.25% solution of bromophenol blue
2.5 ml 2-mercaptoethanol
ddH20 to 10ml.
30:0.8 Acrylamide:bis
0.5 M Tris pH 6.8
10% SDS
10% APS
TEMED
3 M Tris pH 8.8
