本文评述了新近发展起来的以大规模集成流路（IFC）芯片为基础的数字 PCR 技术。指出 PCR 已被证明可用于除外伤和营养不良症以外所有其他疾病的分子诊断。但是传统 PCR 技术有误诊率高的缺点，即便采用传统的数字 PCR 技术，也还会有 2% 以上的差错率。但是当采用 IFC 芯片作数字 PCR 检测时，将可使测量准确度达到 99.75% 以上，并可做绝对测量，因而在各种疾病早期分子诊断方面具有其他方法无法比拟的巨大潜力。
A Very Promising New Analytical and Diagnostic Technology: Large Scale Integrated Fluidic Circuit-PCR
Abstract The newly developed digital polymerase chain reaction(dPCR) technology based on integrated fluidic circuit(IFC) chip is critically reviewed. As was shown that other than injury and dystrophy all the diseases could be di-agnosed with the use of PCR technology. However, the misdiagnosis rate of traditional PCR technology is quite high. An error rate as high as <2% is common even though a traditional digital PCR is used. However, when a digital PCR based on IFC chip is used an accuracy of 99.75% is attainable and even an absolutely quantitative determination is also possible. Therefore, the digital PCR based on IFC chip is a very promising technology for the early molecular diagnosis of various diseases.
Key words Integrated luidic circuit(IFC) chip, Digital PCR, Molecular Diagnosis
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