两种荧光化学原理
●Fluorogenic 5’Nuclease Assay
–An established and proven chemistry for high specificity nucleicacid real-time quantitation
–The introduction of TaqMan®MGB (Minor Groove Binder) probes has produced a turn-key homogeneous chemistry for the detection of Single Nucleotide Polymorphisms (SNPs)
●SYBR®Green 1 Double Stranded DNA Binding Dye Assay
–Ideal for target identification (screening) assays
–A lower-cost alternative when high specificity is not required
TaqManMGB探针
MGB探针的优点
●提高淬灭效率
●减少背景、提高信噪比
●提高探针Tm值
–长15碱基，提高18°C
●缩短探针长度
–最佳范围13-21 碱基
TaqManMGB探针鉴别等位基因
