PCR 酶切位点保护碱基设计

2010-03-02 10:13 · Conrad

酶寡核苷酸序列切割率%2 hr20 hrNde ICCATATGGCCCATATGGGCGCCATATGGCGGGGTTTCATATGAAACCCGGAATTCCATATGGAATTCCGGGAATTCCATATGGAATTCCC000075750000>90>90Nhe IGG

酶	寡核苷酸序列	切割率%
酶	寡核苷酸序列	2 hr	20 hr
Nde I	CCATATGG CCCATATGGG CGCCATATGGCG GGGTTTCATATGAAACCC GGAATTCCATATGGAATTCC GGGAATTCCATATGGAATTCCC	0 0 0 0 75 75	0 0 0 0 <90 <90
Nhe I	GGCTAGCC CGGCTAGCCG CTAGCTAGCTAG	0 10 10	0 25 50
TTGCGGCCGCAA ATTTGCGGCCGCTTTA AAATATGCGGCCGCTATAAA ATAAGAATGCGGCCGCTAAACTAT AAGGAAAAAAGCGGCCGCAAAAGGAAAA	0 10 10 25 25	0 10 10 90 <90
TGCATGCATGCA CCAATGCATTGGTTCTGCAGTT	10 <90	<90 <90
TTAATTAA GTTAATTAAC CCTTAATTAAGG	0 0 0	0 25 <90
GTTTAAAC GGTTTAAACC GGGTTTAAACCC AGCTTTGTTTAAACGGCGCGCCGG	0 0 0 75	0 25 50 <90
GCTGCAGC TGCACTGCAGTGCA AACTGCAGAACCAATGCATTGG AAAACTGCAGCCAATGCATTGGAA CTGCAGAACCAATGCATTGGATGCAT	0 10 <90 <90 0	0 10 <90 <90 0
CCGATCGG ATCGATCGAT TCGCGATCGCGA	0 10 0	0 25 10
CGAGCTCG	10	10
GCCGCGGC TCCCCGCGGGGA	0 50	0 <90
GTCGACGTCAAAAGGCCATAGCGGCCGC GCGTCGACGTCTTGGCCATAGCGGCCGCGG ACGCGTCGACGTCGGCCATAGCGGCCGCGGAA	0 10 10	0 50 75
GAGTACTC AAAAGTACTTTT	10 75	25 75
CCCGGG CCCCGGGG CCCCCGGGGG TCCCCCGGGGGA	0 0 10 <90	10 10 50 <90
GACTAGTC GGACTAGTCC CGGACTAGTCCG CTAGACTAGTCTAG	10 10 0 0	<90 <90 50 50
GGCATGCC CATGCATGCATG ACATGCATGCATGT	0 0 10	0 25 50
AAGGCCTT GAAGGCCTTC AAAAGGCCTTTT	<90 <90 <90	<90 <90 <90
CTCTAGAG GCTCTAGAGC TGCTCTAGAGCA CTAGTCTAGACTAG	0 <90 75 75	0 <90 <90 <90
CCTCGAGG CCCTCGAGGG CCGCTCGAGCGG	0 10 10	0 25 75
CCCCGGGG CCCCCGGGGG CCCCCCGGGGGG TCCCCCCGGGGGGA	0 25 50 <90	0 75 <90 <90

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