微生物学通报 JAN 20,2010,37(1):30～36
一株沙门氏菌拮抗菌的筛选及其在污染土壤原位修复中的应用
宋德显1,2,3 尹睿1,2* 林先贵1,2 王一明1,2 张华勇1,2 王纪杰1,2
(1. 中国科学院南京土壤研究所土壤与农业可持续发展国家重点实验室 江苏 南京 210008)
(2. 中国科学院南京土壤研究所-香港浸会大学土壤与环境联合开放实验室 江苏 南京 210008)
(3. 中国科学院研究生院 北京 100049)
摘 要: 利用无菌滤纸片平板法从沙门氏菌(Salmonella)污染土壤中筛选到一株有效拮抗沙门氏菌的细菌A45, 通过形态学、革兰氏染色和16S rDNA序列同源性分析鉴定为产碱杆菌(Alcaligenes sp.)。温室土培试验和田间原位试验结果都发现, 利用该菌株制备的沙门氏菌拮抗菌剂能显著降低土壤中沙门氏菌数量(P & 0.05), 与对照相比土壤中沙门氏菌数量下降2 3个数量级, 表明该拮抗细菌可应用于沙门氏菌污染土壤的修复。
关键词: 沙门氏菌, 拮抗菌, 土壤污染, 微生物修复, 产碱菌属
Screening of an Antagonistic Bacterial Strain Against Salmonella and Its Application in In-site
Bioremediation of Salmonella-contaminated Soils
SONG De-Xian1,2,3 YIN Rui1,2* LIN Xian-Gui1,2 WANG Yi-Ming1,2
ZHANG Hua-Yong1,2 WANG Ji-Jie1,2
(1. State Key Laboratory of Soil and Sustain able Agriculture, Institute of Soil Science, Chinese Academy of
Sciences, Nanjing, Jiangsu 210008, China)
(2. Joint Open Laboratory of Soil and the Environment, Institute of Soil Science and Hongkong
Baptist University, Nanjing, Jiangsu 210008, China)
(3. Graduate University of Chinese Academy of Sciences, Beijing 100049, China)
Abstract: A bacterial strain, A45, with antagonistic activity against Salmonella was isolated from Salmo-nella-contaminated soils, which was identified as Alcaligenes sp. by checking the individual morphology, colony characteristics and 16S rDNA sequencing. The inhibiting effects of A45 to Salmonella in soils were investigated by field and greenhouse pot experiments respectively. The amounts of Salmonella in soils de-creased up to three orders of magnitude after spraying A45 into soils both under greenhouse and field condi-tions. It suggested that it might be an effective, economical and environmentally friendly approach to apply A45 for in-site remediation of pathogens-contaminated soils.
Keywords: Salmonella, Antagonistic bacterium, Soil pollution, Bioremediation, Alcaligenes sp.
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