摘要： 本文主要报道在1.2(?)高分辨率利用限制立体化学参数的倒易空间最小二乘技术对胰岛素的非氢原子进行各向异性温度因子修正后的结果。在修正后的电子密度图上,少数原子电子密度的各向异性分布有明显表现;80％的氢原子的电子密度有不同程度的反映;修正建立的包括水和溶剂体系,氢原子和氢键在内的胰岛素分子的精确结构模型,为各种胰岛素类似物结构的分析和比较,以及结构与功能关系的研究提供了可靠的,精度的参照和基础。
常文瑞,戴金璧,张季平,谢殿霖,D.Stuart,R.Todd,梁栋材,邝宝
作者单位：中国科学院生物物理研究所,北京；北京大学生物系,北京
keywords： THE CRYSTAL STRUCTURE OF PORCINE INSULIN REFINED WITH TEMPERATURE FACTORS AT 1.2 A RESOLUTION——STRUCTURE INFORMATION FROM TEMPERATURE FACTORS, WATER MOLECULES, HYDROGEN ATOMS AND HYDROGEN BONDS
Abstract: The results of refinement of porcine insulin structure with anisotropic temperature factors using the reciprocal space least squares method, restrained by stereochmical parameters,are described in this paper. The anisotropic distrbuti-on of electron density of some atoms and the density peaks of 80% hydrogenatoms can be seen clearly on the final maps. The refined structure model including waters,bulk solvent and hydrogen bonds provide a reliable high precision reference and a basis for comparing the structures of analogues of insulin and for studying the relationship between function and structure of it.
