Abstract： The selenium-containing phycocyanin from the selenium-rich algae (Spirulina platensis) has been crystallized in two crystal forms by the hanging-drop vapor diffusion techniques. A chromatographic procedure of gel filtration and anion exchange was used for purification. Form I crystal with space group P21 and cell parameters a =108.0 ?, b = 117.0 ?, c = 184.0 ?, b = 90.2° and 12(ab) units in the asymmetric unit was obtained by using (NH4)2SO4 as precipitant. These crystals diffract up to 2.8 ?. Form II crystal obtained by using PEG4000 as precipitant belongs to space group P63 with unit cell constants a = 155.0 ?, c = 40.3 ?, g =120.0° and one(ab) unit in the asymmetric unit. The crystals diffract beyond 2.9 ?. The possible stacking forms of phycocyanin molecules in the first crystal form were discussed.
作者： 李樂農 郭寶江 張季平 江濤 常文瑞 梁棟材 LI Lenong GUO Baojiang Zhang Jiping JIANG Tao CHANG Wenrui LIANG Dongcai
作者單位： 李樂農,郭寶江,LI Lenong,GUO Baojiang(Biotechnology Research Institute, South China Normal University, Guangzhou 510631, China)
張季平,江濤,常文瑞,梁棟材,Zhang Jiping,JIANG Tao,CHANG Wenrui,LIANG Dongcai(National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China)
