生物芯片微阵列荧光图产生方法与过程
由于拿到的只是已经生成好的微阵列图片。在对其分析与图像处理时还是不能够清楚地把握其性质与优劣评估，之前只是自我猜测。比如每个斑点的定位，斑点内不均匀灰度值的读取，缺失斑点的处理等等。。于是花了一些时间在网上搜了一些关于微阵列产生过程与方法的材料：
先看国内：
[基因芯片]基因芯片技术知识概要 https://www.css2.com.cn/2006/9-19/112410.html
DNA芯片和生物芯片软件 https://www.bio-soft.net/chip.html
再看国外：（想了解更多可以点击查看图片所在的网址）
1.https://www.mun.ca/biology/scarr/cDNA_microarray_Assay_of_Gene_Expression.html
Principle of cDNA microarray assay of gene expression
A microarray is a set of short Expressed Sequence Tags (ESTs) made from a cDNA library of a set of known (or partially known) gene loci. The ESTs are spotted onto a cover-slip-sized glass plate, shown here as a 8x12 array. In practice, microarrays of many thousand ESTs are possible.
mRNA transcriptomes are prepared from the tissue of an experimental treatment or condition. For example, fish fed a high-protein diet, or an individual with breast cancer. Complementary DNA (cDNA) reverse transcripts are prepared and labelled with a [red] fluorescent dye. A control library is constructed from an untreated source [standard diet, or non-cancerous tissue], and labelled with a different fluroescent [green] dye. The experimental and control libraries are hybridized to the microarray. Dual-channel laser excitation excites the corresponding dye, which fluoresces proportional to the degree of hybridization that has occurred. Relative gene expression is measured as the ratio of the two fluorescences: "up-regulation" of the experimental transcriptome relative to the control will be visualized as a red "pseudo-colour," "down-regulation" shows as green, and constitutive expression_r(1:1 versus control) as a neutral black. Intensity of color is proportional to the expression differential.
2.https://www.genome.gov/Pages/Hyperion/DIR/VIP/Glossary/Illustration/microarray_technology.cfm?key=microarray%20technology
3.Image:Microarray printing.ogg View the VIDEO： https://commons.wikimedia.org/wiki/Image:Microarray_printing.ogg
一个基因主板被机器人打印出来。（要专门播放器）
4.Microarray Facility https://biomedical.ucsc.edu/Microarray.html
5.https://www.biogate.it/microarrays/microarrays.html
6.https://www.biogate.it/microarrays/images/microarray_principle_en.jpg
7.Microarray information flow （他的相册里还有几张不错的流程图）
8.https://www.stathis.co.uk/art/infographics/infographics-gallery/?pid=96
