摘要：某一特定基因被“关闭”的基因剔除小鼠是基因功能分析的关键。这篇论文报告了一个重要资源，即一种高吞吐量的基因定位管道，它已在C57BL/6胚胎干细胞系中生成了数以千计的条件突变，适合为进行大规模基因表现型分析的研究工作培育突变小鼠。该策略也适用于大鼠和人的干细胞，为确定由哺乳动物基因组编码的所有基因的功能提供了一个基础。
小鼠基因敲除技术平台
生物探索推荐英文摘要
Nature vol.474 (7351), (16 Jun 2011)
A conditional knockout resource for the genome-wide study of mouse gene function
Abstract：Gene targeting in embryonic stem cells has become the principal technology for manipulation of the mouse genome, offering unrivalled accuracy in allele design and access to conditional mutagenesis. To bring these advantages to the wider research community, large-scale mouse knockout programmes are producing a permanent resource of targeted mutations in all protein-coding genes. Here we report the establishment of a high-throughput gene-targeting pipeline for the generation of reporter-tagged, conditional alleles. Computational allele design, 96-well modular vector construction and high-efficiency gene-targeting strategies have been combined to mutate genes on an unprecedented scale. So far, more than 12,000 vectors and 9,000 conditional targeted alleles have been produced in highly germline-competent C57BL/6N embryonic stem cells. High-throughput genome engineering highlighted by this study is broadly applicable to rat and human stem cells and provides a foundation for future genome-wide efforts aimed at deciphering the function of all genes encoded by the mammalian genome.
