MCMV 和SCMV复合侵染玉米引起玉米组织中siRNAs的积累
RNA沉默是真核生物中调控基因表达的一种保守机制，它在植物生长发育和抗病毒中发挥着重要的作用。RNA病毒进入寄主植物细胞后，其复制中间复合体和单链基因组RNA的高级结构形成的双链RNA可以被寄主植物DCL蛋白识别，加工产生21-24-nt的初级来源于病毒的siRNA（vsiRNA）。初级vsiRNA装载到特异AGO蛋白中形成RISC复合体，根据碱基互补配对的原则识别其靶标并主要以切割的方式降解靶标。病毒基因组是vsiRNA最主要的靶标，被切割后的病毒RNA可以被RDR识别，并将其加工成双链RNA，再一次被DCL蛋白切割形成次级vsiRNA。初级和次级vsiRNA没有本质上的区别，都可以作用于靶标，发挥抗病毒的功能。玉米褪绿斑驳病毒（MCMV）与侵染玉米的马铃薯Y病毒科病毒，如玉米矮花叶病毒（MDMV）、小麦条纹花叶病毒（WSMV）和甘蔗花叶病毒（SCMV），复合侵染时引起玉米致死性坏死（MLN），对玉米产量造成很大的威胁。虽然有报道称马铃薯Y病毒属病毒编码的沉默抑制子HC-Pro可以增加异源病毒的致病性和积累量，并且MCMV与马铃薯Y病毒科病毒复合侵染时积累量显著增加，但是，关于它们复合侵染的机制研究却很少。我们准备以MCMV和SCMV复合侵染为例，从RNA沉默的角度初步探索其复合侵染的机制。该研究中miRNA 测序服务由上海伯豪生物技术有限公司提供。详细内容： https://www.shbio.cn/news/research/3046.html
2016年3月：上海伯豪客户应用案例精选
1 Wu W, Hai Y, Chen L, Liu RJ, Han YX, Li WH, Li S, Li S,Wu XR. Deguelin-induced blockade of PI3K/protein kinase B/MAP kinase signaling in zebrafish and breast cancer cell lines is mediated by down-regulation of fibroblast growth factor receptor 4 activity. Pharmacology Research & Perspectives.2016.
2 Song X, Gao T, Wang N, Feng Q, You X, Ye T, Lei Q, Zhu Y, Xiong M, Xia Y, Yang F, Shi Y, Wei Y, Zhang L, Yu L. Selective inhibition of EZH2 by ZLD1039 blocks H3K27methylation and leads to potent anti-tumor activity in breast cancer. Sci Rep.2016.
3 Zheng QF1, Zhang JY, Wu JS, Zhang Y, Liu M, Bai L, Zhang JY, Zhao J, Chen Y, Duan ZP, Zheng SJ. Upregulation of miRNA-130a Represents Good Prognosis in Patients With HBV-Related Acute-on-Chronic Liver Failure: A Prospective Study. Medicine (Baltimore). 2016 ,95(6):e2639.
4 Mao H, Yu L, Li Z, Liu H, Han R. Molecular evolution and gene expression differences within the HD-Zip transcription factor family of Zea mays L. Genetica.2016.
5 Xia Z, Zhao Z, Chen L, Li M, Zhou T, Deng C, Zhou Q, Fan Z. Synergistic infection of two viruses MCMV and SCMV increases the accumulations of both MCMV and MCMV-derived siRNAs in maize. Sci Rep. 2016 ,6:20520.
6 Shang FF, Xia QJ, Liu W, Xia L, Qian BJ, You L, He M, Yang JL, Wang TH. miR-434-3p and DNA hypomethylation co-regulate eIF5A1 to increase AChRs and to improve plasticity in SCT rat skeletal muscle. Sci Rep. 2016 ,6:22884.
7 Tian J, Song Y, Du Q, Yang X, Ci D, Chen J, Xie J, Li B, Zhang D. Population genomic analysis of gibberellin-responsive long non-coding RNAs in Populus. J Exp Bot.2016.
8 Sun Y, Cai J, Yu S, Chen S, Li F, Fan C. MiR-630 Inhibits Endothelial-Mesenchymal Transition by Targeting Slug in Traumatic Heterotopic Ossification. Sci Rep. 2016,6:22729.
9 Dong R, Shen Z, Zheng C, Chen G, Zheng S. Serum microRNA microarray analysis identifies miR-4429 and miR-4689 are potential diagnostic biomarkers for biliary atresia. Sci Rep. 2016,6:21084.
