北京生命科学研究所邓兴旺实验室在Plant Molecular Biology杂志上在线发表题为 “ Genome-wide profiling of histone H3 lysine 9 acetylation and dimethylation in Arabidopsis reveals correlation between multiple histone marks and gene expression”的文章。 该文章通过ChIP-chip技术, 对拟南芥基因组水平组蛋白修饰与基因表达之间的关系进行了研究。
组蛋白H3的赖氨酸9位点可以被乙酰化及单、二或三甲基化，这些组蛋白的修饰状态对基因的表达以及染色质的组织结构有一定的影响。在拟南芥中，H3K9ac几乎毫无例外地与转录激活相关，而H3K9me2则主要位于组成型异染色质区。本文报道了拟南芥全基因组组蛋白H3K9ac和H3K9me2修饰的图谱，结果表明H3K9ac修饰5206个非TE编码基因和321 TE编码基因，而H3K9me2修饰2281个TE基因和1112个非TE编码基因。H3K9ac修饰集中在基因的5′末端，在ATG位置有一个高峰，而H3K9me2修饰则覆盖基因的整个编码区。 H3K9ac修饰的基因的表达活性相对较高，而H3K9me2则多分布在表达活性较低的基因。对本文中H3K9ac和H3K9me2的全基因组修饰的图谱与已经报道的H3K27me3和DNA甲基化修饰图谱一起研究发现，同一基因上的组蛋白多个位点修饰和基因的表达活性之间存在相关性。
原始出处：
Plant Molecular Biology DOI:10.1007/s11103-009-9594-7
Genome-wide profiling of histone H3 lysine 9 acetylation and dimethylation in Arabidopsis reveals correlation between multiple histone marks and gene expression
Junli Zhou1, 2 , Xiangfeng Wang1, 2 , Kun He2, 3 , Jean-Benoit F. Charron2 , Axel A. Elling2 and Xing Wang Deng1, 2, 3
(1) National Institute of Biological Sciences, 7 Science Park Road, Zhongguancun Life Science Park, 102206 Beijing, People’s Republic of China
(2) Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520, USA
(3) Peking-Yale Joint Center of Plant Molecular Genetics and Agrobiotechnology, College of Life Sciences, Peking University, 100871 Beijing, People’s Republic of China
Lysine residue 9 of histone H3 can either be acetylated or mono-, di-, or tri-methylated. These epigenetic states have a diverse impact on regulating gene transcriptional activity and chromatin organization. H3K9ac is invariably correlated with transcriptional activation, whereas H3K9me2 has been reported to be mainly located in constitutive heterochromatin in Arabidopsis. Here, we present epigenetic landscapes for histone H3 lysine 9 acetylation (H3K9ac) and dimethylation (H3K9me2) in Arabidopsis seedlings. The results show that H3K9ac targeted 5,206 non-transposable element (non-TE) genes and 321 transposable elements (TEs), whereas H3K9me2 targeted 2,281 TEs and 1,112 non-TE genes. H3K9ac was biased towards the 5′ end of genes and peaked at the ATG position, while H3K9me2 tended to span the entire gene body. H3K9ac correlated with high gene expression, while H3K9me2 correlated with low expression. Analyses of H3K9ac and H3K9me2 with the available datasets of H3K27me3 and DNA methylation revealed a correlation between the occurrence of multiple epigenetic modifications and gene expression. Genes with H3K9ac alone were actively transcribed, while genes that were also modified by either H3K27me3 or DNA methylation showed a lower expression level, suggesting that a combination of repressive marks weakened the positive regulatory effect of H3K9ac. Furthermore, we observed a significant increase of the H3K9ac modification level of selected target genes in hda19 (histone deacetylase 19) mutant seedlings, which indicated that HDA19 plays an important role in regulating the level of H3K9ac and thereby influencing the transcriptional activity in young seedlings.
Electronic supplementary material The online version of this article (doi:10.1007/s11103-009-9594-7) contains supplementary material, which is available to authorized users.
