Nature:唐氏综合症的抗肿瘤机制

2010-06-10 00:00 · ann

唐氏综合症由21号染色体一个额外版本的存在(一种被称为“三体性”的状态)引起,而且人们知道某些肿瘤的生长在这种遗传病中被抑制。 用一个唐氏综合症小鼠模型所做的一项研究指出了一个抗肿瘤机制:通过四个基因的过度 表达来抑制肿瘤血管生成,这四个基因分别是两个假设的抗血管生成基因(ADA

唐氏综合症由21号染色体一个额外版本的存在(一种被称为“三体性”的状态)引起,而且人们知道某些肿瘤的生长在这种遗传病中被抑制。

用一个唐氏综合症小鼠模型所做的一项研究指出了一个抗肿瘤机制:通过四个基因的过度 表达来抑制肿瘤血管生成,这四个基因分别是两个假设的抗血管生成基因(ADAMTS1 和 ERG10)及两个以前没有与血管生成联系在一起的新的内皮细胞特定基因(JAM-B12和 PTTG1IP)。

推荐原文出处:

Nature doi:10.1038/nature09106

Tumour angiogenesis is reduced in the Tc1 mouse model of Down’s syndrome

Louise E. Reynolds1, Alan R. Watson1,14, Marianne Baker1,14, Tania A. Jones3, Gabriela D’Amico1, Stephen D. Robinson1, Carine Joffre2, Sarah Garrido-Urbani5, Juan Carlos Rodriguez-Manzaneque6, Estefanía Martino-Echarri6, Michel Aurrand-Lions7, Denise Sheer3, Franca Dagna-Bricarelli8, Dean Nizetic4, Christopher J. McCabe9, Andrew S. Turnell10, Stephanie Kermorgant2, Beat A. Imhof5, Ralf Adams11, Elizabeth M. C. Fisher12, Victor L. J. Tybulewicz13, Ian R. Hart2 " Kairbaan M. Hodivala-Dilke1

Down’s syndrome (DS) is a genetic disorder caused by full or partial trisomy of human chromosome 21 and presents with many clinical phenotypes including a reduced incidence of solid tumours1, 2. Recent work with the Ts65Dn model of DS, which has orthologues of about 50% of the genes on chromosome 21 (Hsa21), has indicated that three copies of the ETS2 (ref. 3) or DS candidate region 1 (DSCR1) genes4 (a previously known suppressor of angiogenesis5, 6) is sufficient to inhibit tumour growth. Here we use the Tc1 transchromosomic mouse model of DS7 to dissect the contribution of extra copies of genes on Hsa21 to tumour angiogenesis. This mouse expresses roughly 81% of Hsa21 genes but not the human DSCR1 region. We transplanted B16F0 and Lewis lung carcinoma tumour cells into Tc1 mice and showed that growth of these tumours was substantially reduced compared with wild-type littermate controls. Furthermore, tumour angiogenesis was significantly repressed in Tc1 mice. In particular, in vitro and in vivo angiogenic responses to vascular endothelial growth factor (VEGF) were inhibited. Examination of the genes on the segment of Hsa21 in Tc1 mice identified putative anti-angiogenic genes (ADAMTS18, 9and ERG10) and novel endothelial cell-specific genes11, never previously shown to be involved in angiogenesis (JAM-B12 and PTTG1IP), that, when overexpressed, are responsible for inhibiting angiogenic responses to VEGF. Three copies of these genes within the stromal compartment reduced tumour angiogenesis, explaining the reduced tumour growth in DS. Furthermore, we expect that, in addition to the candidate genes that we show to be involved in the repression of angiogenesis, the Tc1 mouse model of DS will permit the identification of other endothelium-specific anti-angiogenic targets relevant to a broad spectrum of cancer patients.

Adhesion and Angiogenesis Laboratory, Barts Institute of Cancer, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, John Vane Science Centre, Charterhouse Square, London EC1M 6BQ, UK

Centre for Tumour Biology, Institute of Cancer, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, John Vane Science Centre, Charterhouse Square, London EC1M 6BQ, UK

Neuroscience Centre, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, Institute of Cell and Molecular Sciences, 4 Newark Street, London E1 2AD, UK

Paediatrics Centre, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, Institute of Cell and Molecular Sciences, 4 Newark Street, London E1 2AD, UK

Department of Pathology and Immunology, Centre Medical Universitaire, University of Geneva Medical School (CMU), rue Michel Servet 1, CH-1211 Geneva, Switzerland

GENYO, Avenida Del Conocimiento, s/n Armilla 18100, Granada, Spain

INSERM, 27, Boulevard Lei Roure, 13009 Marseille, France

Human Genetics Institute, Galliere Hospital, Via Volta 10, 16128 Genoa, Italy

School of Clinical and Experimental Medicine, University of Birmingham, Birmingham B15 2TT, UK

School of Cancer Sciences, University of Birmingham, Birmingham B15 2TT, UK

Max Planck Institute for Molecular Biomedicine, R?ntgenstrasse 20, D-48149 Münster, Germany

Department of Neurodegenerative Disease, UCL Institute of Neurology, Queen Square, London WC1N 3BG, UK

Division of Immune Cell Biology, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK

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