微生物学通报 MAR 20,2010,37(3):473～478
一株微囊藻毒素降解辅助菌的分离和鉴定
李现尧 刘晓文 史全良*
(苏州大学基础医学与生物科学学院 江苏 苏州 215123)
摘 要: 以从太湖蓝藻中提取的微囊藻毒素作为微囊藻毒素降解菌的筛选物质, 通过稀释平板涂布法从腐烂的蓝藻中富集分离到一菌株, 经形态特征、生理生化特征和16S rDNA 序列分析将该菌株(GenBank 序列登录号为GQ143751)鉴定为藤黄微球菌(Micrococcus luteus); 微囊藻毒素降解实验结果表明该菌株几乎不能降解微囊藻毒素, 但可以明显促进一株微囊藻毒素降解菌微嗜酸寡养单胞菌(Stenotrophomonas acidaminiphila)对微囊藻毒素的降解能力, 将筛选菌株与微嗜酸寡养单胞菌混合培养, 混合菌对微囊藻毒素的降解能力比微嗜酸寡养单胞菌单独培养时提高66.7%。
关键词: 微囊藻毒素, 生物降解, 辅助菌, 藤黄微球菌
Isolation and Identification of a Microcystin-degrading Assistant Bacterium
LI Xian-Yao LIU Xiao-Wen SHI Quan-Liang*
(School of Basic Medicine and Biological Sciences, Soochow University, Suzhou, Jiangsu 215123, China)
Abstract: A bacterial strain was enriched and isolated from rotten cyanobacteria of Taihu Lake by dilu-tion-plate method, using microcystins as selective material of microcystin-degrading bacteria, which was ex-tracted and purified from cells of cyanobacteria. Based on morphological, physiological characteristics and 16S rDNA sequence analysis, the strain is preliminarily identified as Micrococcus luteus (GenBank acces-sion number is GQ143751). The results of microcystin-degrading experiments show that the strain could hardly degrade microcystins but could enhance the microcystin-degrading capability of another strain Steno-trophomonas acidaminiphila. When the strain is mixed with Stenotrophomonas acidaminiphila, the micro-cystin-degrading capability of mixed bacteria is improved 66.7% compared with Stenotrophomonas acida-miniphila.
Keywords: Microcystins, Biodegradation, Assistant bacteria, Micrococcus luteus
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